The drug has undergone extensive field tests on different crops (rye, triticale, winter and spring wheat, barley, rice, peas, broad beans, corn; forage grasses - coarse millet, Shattercane, spring oilseed rape) in different regions of Russia (Ulyanovsk, Kurgan, Ryazan, Penza regions, Krasnodar Territory).
According to the research of the scientists of Ulyanovsk State Agricultural Academy (V. Kostin, O. Kostin, V. Isaichev, 2006) it has been found out that:
In the Kuban State Agrarian University (A. Barchukova, N. Chernysheva) there has been studied the Melafen effect on germination of seeds of Krasnodar 99 winter wheat variety and Mikhailo winter barley variety, yield and grain quality.
In Ryazan State Agricultural Academy (S. Chepko, L. Dolgova, V. Polozhentsev, 2006) there has been studied the effect of Melafen on winter rye and winter triticale.
There has been discovered a positive influence of Melafen on seed treatment before sowing. Germination increased by 5-8%.
Application of Melafen on shoots helped reduce the lodging of winter rye by 12-21% (drug concentration 1*10-5% - 10-'9 %).
The plantings of winter triticale treated with Melafen were not beaten down. Treatment of shoots with the drug helped increase the resistance of plants to fungal diseases.
Interesting results were obtained during treatment of potato tubers with Melafen before planting (V. Polozhentsev, E. Polozhentseva, O. Polozhentsev, E. Trushin, 2006). The most effective was the concentration of 1*10-8 %. When using Melafen in the required concentration potato yield increased by 10.1%. 63.6% of the yield consisted of large and mid fractions.
There have been carried out in-depth studies of plant protein tyrosine phosphorylation induced by Melafen (F. Karimova, S. Vanyushina, E. Fedina, F. Mudarisov, 2006). These data indicate the important role of protein tyrosine phosphorylation in the regulation of metabolic pathways of plant cells with Melafen, in particular, reactions of photosynthetic assimilation of carbon in plant chloroplasts. A change of the level of protein tyrosine phosphorylation with Melafen indicates high efficiency of Melafen in regulation of metabolism in plant cells by tyrosine kinase signaling system.
In assessing the environmental impact of chemicals, biological indicators related to effective fertility of the soil are used. An integrated detector of the level of human influence characterizing the functional activity of the soil microflora is its enzymatic activity (A. Svirskene, 2003).
The staff of the Kazan State University (N. Zakharov, Z. Siraeva, I. Demidova, etc., 2006) have found out that Melafen in the concentration range of 10-6 -10-7 % does nothave any negative impact on the enzymatic activity of soil and in some cases slightly increases the activity of enzymes (ureases and proteases) involved in the transformation of organic nitrogen compounds. Melafen concentration of 10-7 % significantly stimulates the process of potential nitrogen fixation by the 7th and 15th days.
Currently, there are ongoing tests of Melafen on many crops as well as its use in ultra-low doses (A. Barchukova, N. Chernysheva , S. Volkova, S. Fattakhov, V. Reznik, A. Konovalov, 2008).